Crystalline Suspension of Bovine Zinc Des-AlaB30-Insulin with Prolonged Hypoglycaemic Activity
نویسندگان
چکیده
The reversible reaction of cyclohexane-1,2-dione with the guanidino function of Arg-B22 in bovine insulin gave the dihydroxycyclohexylene [DHCH]-Arg-B22-insulin complex (I) which, after selective tryptic cleavage at the Lys-B29 residue and regeneration of the guanidino group, afforded bovine des-AlaB30-insulin (III) in an overal 85.80/o yield. The truncated insulin III was converted into the regular zinc salt and, under conditions that favour binding of more than 2 Zn atoms per insulin hexamer unit, into a crystalline suspension. When tested for their hypoglycaemic activity, the two forms of III exhibited significantly different durations of the effect in mice: the blood glucose curves obtained for the III-Zn salt and the III-crystalline suspension were indistinguishable from those exerted by regularand long-acting bovine zinc insulin, respectively.
منابع مشابه
Prolonged Laboratory Maintenance of Toxoplasma gondii Tachyzoites in Co-cultivation with the Bovine Theileria annulata-Infected Lymphoblastoids
Background and Aims: In most of the studies, Toxoplasma gondii is maintained in laboratory mice or studied in vitro using non-lymphoid cell lines or primary mouse macrophages. The target of our research was to design a new axenic culture of Toxoplasma gondii tachyzoites to providing a sufficient quantity of them. Material and Methods: Theileria annulata-infected lymphoblastoids, which had been...
متن کاملMotor aphasia due to prolonged hypoglycaemic coma in a patient with insulin-dependent diabetes mellitus.
A 45-year-old insulin-dependent diabetic man was in a hypoglycaemic coma for one month but recovered after continuous infusion of glucose and insulin. An isolated neurological deficit, motor aphasia, persisted after recovery from the coma. Repeated computerized tomography did not demonstrate any abnormal findings attributable to coma or aphasia. Precise follow-up examinations of aphasia showed ...
متن کاملAntidiabetic Activity of Zinc Oxide and Silver Nanoparticles on Streptozotocin-Induced Diabetic Rats
The use of nanoparticles in medicine is an attractive proposition. In the present study, zinc oxide and silver nanoparticles were evaluated for their antidiabetic activity. Fifty male albino rats with weight 120 ± 20 and age 6 months were used. Animals were grouped as follows: control; did not receive any type of treatment, diabetic; received a single intraperitoneal dose of streptozotocin (100...
متن کاملThe Catabolism of I-131-insulin by Rat Lens Homogenates.
The ability of a rat lens homogenate to degrade radioiodinated zinc insulin into fractions which now are TCA soluble was used as a criterion of proteolysis. The assay method is extremely simple, rapid, and sensitive. Proteolytic activity is linearly related to the amount of lens homogenate added. The lens enzyme has a pH optimum of approximately 7.2, a temperature maximum near 50° C, and is ina...
متن کاملA clinical comparison of modified insulins.
The shortcomings of standard protamine zinc insulin when used alone in a single daily injection for the treatment of so-called "severe" diabetes are well known (1-4). Post-prandial glycosuria, nocturnal hypoglycemia, or both, are common and are directly attributed to its slow, gradual but variable rate of absorption from the subcutaneous depot (5). Consequently, increasing attention has been di...
متن کامل